Abstract: To address problems of disconnect between theory and cutting-edge applications and insufficient cultivation of students' scientific research thinking in undergraduate food microbiology experimental teaching, this course has drawn upon research conducted by our faculty in field of photodynamic antibacterial properties of ZIF@CCM nanocomposites．An exploratory experimental project was designed to integrate foundational microbiology with interdisciplinary technologies, resulting in a teaching program that combines fundamental, intersectional, and inquiry-based elements．Experiment content covered core skills such as Escherichia coli cultivation, MIC/MBC determination, K⁺ leakage detection, and singlet oxygen measurement, while incorporating cutting-edge knowledge of nanomaterials and photodynamic antibacterial technology．Teaching optimization has been advanced through a five-dimensional strategy: constructing a logical framework that clearly-defined objectives-connected principles-linked practices, highlighting experiments' exploratory nature through variable control and control group design, optimizing results presentation with intuitive charts and guiding questions, adding error analysis to strengthen skill reflection, and simplifying complex theories to match undergraduate cognitive levels．This experiment has helped students establish a scientific logical chain among material properties, experimental operations, and antibacterial phenomena, thereby enhancing their interdisciplinary thinking and scientific research practical abilities．