Abstract: Mixed noise in high-density stacked laying hen’s house was collected by sound level meter.Spectrum features of mechanical noise of each production system in laying hen’s house were obtained by using audio analysis software Praat 5.3，acoustic characteristic parameters and spatial distribution characteristics of mixed noise were analyzed.Results of investigation on mixed noise of high-density stacked laying hen’s house demonstrated that influence of mechanic noise to acoustic environmental monitoring was arranged as follows： mechanically ventilated system，manure collection system，food filling system，feeding system and egg collected system.Significant difference（P<0.05）was exsisted between prodcution systems with acoustic characteristic parameters.Weighted sound pressure A of laying hen’s house was 69.0～75.0 dB for inner environment.Variations of mixed noise with vertical and horizontal direction of laying hen’s house didn’t agree with each other，and spatial distribution of acoustic characteristics was also significantly differed（P<0.05）.Measurement and analysis of mixed noise with high-density stacked cage system is very important for noise management and sound quality evaluation of production systems of laying hen’s house.To some extent，reasonable adjustments to running program and operating time of mechanic equipment could reduce noise and body injury to laying hens with a guarantee to normal breeding conditions.