Abstract: The inoculation steps of bag-grown edible fungus liquid strain inoculation machine are divided into four parts：feeding，punching，inoculation and filming.Intelligent control system based on PLC was introduced which realized the four in one functions of feeding，drilling，inoculation and film sticking of irregular shape Lentinus edodes sticks.Practice has shown that control system of edible fungus liquid inoculation machine was stable，reliable，flexible in operation and complete in functions.