叶酸含量测定在食品生物化学实验教学中的实践

严慧玲; 郭新波; 刘金钏

doi:10.19998/j.cnki.2095-1795.2022.06.025

叶酸含量测定在食品生物化学实验教学中的实践

doi: 10.19998/j.cnki.2095-1795.2022.06.025

华南理工大学轻工与食品国家级实验教学示范中心，广东广州 510640

基金项目: 华南理工大学探索性实验项目（x2sk-C9212270）

详细信息

作者简介:
严慧玲，硕士，主要从事食品生物化学研究　E-mail：fehlyan@scut.edu.cn

郭新波，通信作者，博士，副教授，主要从事食品分子营养学研究　E-mail：guoxinbo@scut.edu.cn

中图分类号: G642.0
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- 被引次数: 0
出版历程
- 收稿日期: 2021-12-10
- 修回日期: 2022-02-27
- 出版日期: 2022-06-20

Practice of Folic Acid Determination in Food Biochemistry Experiment

Experimental Teaching Demonstration Center of Light Industry and Food science，South China University of Technology， Guangzhou Guangdong 510641，China

摘要

摘要:
为更好地培养综合型人才，打破不同实验课程之间的孤立性，首次将叶酸提取与测定纳入本科生食品生物化学实验教学中。以生活中常见的食品为原材料，通过酶解等方法提取叶酸，利用微生物法进行检测。该实验知识点覆盖面广，将食品生物化学与微生物学有机结合起来，可帮助学生实现食品生物化学与微生物学理论体系的融会贯通。前期投入少，无需特殊设备，可作为高校食品专业的综合性实验项目。
- 叶酸 /
- 实验教学 /
- 食品生物化学
Abstract:
To better cultivate comprehensive talents and break isolation between different traditional experimental courses, extraction and determination of folic acid were brought into experiment course of food biochemistry for undergraduates for the first time．Based on ordinary food as raw material, folic acid was extracted by enzymolysis and detected by microbial method．Combination courses of Food Biochemistry and Microbiology will extend students' experimental knowledge by a large margin and help them to build up integral theoretical system of Food Biochemistry and Microbiology．This experiment course, with low cost, could be adopted as a comprehensive experiment project of food specialty in colleges and universities．
- folic acid /
- experimental teaching /
- food biochemistry

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图 1 实验流程

Figure 1. Experimental process

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表 1 实验步骤及对应知识点

Table 1. Experimental steps and knowledge points

实验步骤	对应知识点
叶酸的提取	叶酸：微溶于水，对热、光、酸性溶液均不稳定，在中性及碱性溶液中对热稳定 α-淀粉酶：水解淀粉内部的α-1,4-糖苷键，水解产物为糊精、低聚糖和单糖，释放与淀粉大分子结合的叶酸蛋白酶：将大分子蛋白质水解成小分子肽和氨基酸，释放与蛋白质大分子结合的叶酸叶酸轭合酶：对叶酸进行水解，使各种形式叶酸均转变成单谷氨酸叶酸或含2个或3个谷氨酸尾的叶酸
菌悬液的制备	培养基配制及包扎、灭菌、平板画线、无菌操作
叶酸的检测	无菌操作、菌体密度检测

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