亳菊组织培养技术
Tissue Culture of Chrysanthemum morifolium Ramat
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摘要: 以亳菊茎段作外植体,进行离体培养研究。丛生芽转入到MS培养基上进行继代培养扩大增殖,取长2~3 cm的芽苗在MS+NAA(0.05 mgL)培养基上生根并形成完整植株。MS+6-BA(2.0 mg/L)+NAA(0.1 mg/L)的培养基适宜于诱导侧芽萌发丛生芽。Abstract: According to the plant tissue culture instructions of technical operation request,the explant originated from lateral bud of dendranthem a morifolum was sterilized in surface by the double sterilizing method.The clustered bud was cultured continued on the medium of MS.The shoots of 2~3 cm length were rooted and grown plants in the culturing medium of MS+NAA(0.05 mgL).The suitable medium for the tissue culture was selected according to the quantity of shoots reproduced MS+6-BA(2.0 mgL)+NAA(0.1 mgL)is suitable for the lateral bud becoming clustered bud.
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