Experimental teaching design for microbial VBNC state induction and detection

Relying on scientific research achievements in field of food-borne microbial pollution control, combined with reform needs of food microbiology experimental teaching, an exploratory experimental project jointed production and education was designed around industrial problem of beer spoilage bacteria being easily missed inspection due to their viable of living but non-culturable state．Using typical beer spoilage bacteria（L．casei）as experimental subjects, under technological conditions of brewing beer, process of beer spoilage bacteria forming a viable but nonculturable state was simulated using beer continuous passage method and low-temperature storage method, and molecular methods were used for detection．This project have a certain significance in cultivating undergraduate students' practical application and research literacy．