Zeng Weijie, Liang Shuming, Gao Kaiwen. Fast Determination of Seven Ginsenosides in Abstract of Panax Ginseng by HPLC[J]. AGRICULTURAL ENGINEERING, 2012, 2(3): 47-50.
Citation: Zeng Weijie, Liang Shuming, Gao Kaiwen. Fast Determination of Seven Ginsenosides in Abstract of Panax Ginseng by HPLC[J]. AGRICULTURAL ENGINEERING, 2012, 2(3): 47-50.

Fast Determination of Seven Ginsenosides in Abstract of Panax Ginseng by HPLC

  • The method for the determination of seven ginsenosides in abstract of Panax ginseng by Kinetex core-shell column was established.The separation was performed on Phenomenex Kinetex C18 100A(50 mm×4.6 mm,2.6μm).The mobile phase consisted of acetonitrile(A)-water(B)with Linear gradient elution;at a flow rate of 1.8mL·min-1:the warelength of detector was 205nm.The column temperature was at room temperature.The calibration curve showed good linearity.The relative standard deviation of ginsenoside Rg1、Re、Rf、Rb1、Rc、Rb2 and Rd were 2.5%、2.9%、3.8%、1.5%、3.6%、1.9% and 2.3%.The recovery were 102.6%、99.6%、98.7%、101.3%、99.2%、98.5% and 101.8%.It has the advantage of simplicity,fast determination and less reagent consumed in determination of ginsenoside Rg1、Re、Rf、Rb1、Rc、Rb2 and Rd in abstract of panax ginseng.
  • loading

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return