Establishment of Regeneration System in Vitro Culture of Stevia rebaudiana Bertoni

Establishment method of tissue culture and regeneration system of Stevia rebaudiana Bertoni were studied by using leaves and stem as explants.Results indicated that stem used as explant was superior to leaf in tissue culture.Optimum medium for stem to induce callus and multiple shoots was MS+0.5 mgL 6-BA+0.5 mgL NAA+3% sucrose+0.7% agar.Based on orthogonal experiment，most important factor on inducing cluster buds was macroelement，next was 6-BA，sucrose，and the least was NAA.Optimum medium for rooting was MS+0.25 mgL IBA+0.2 mgL NAA+3% sucrose+0.7% agar+0.3 mgL activated carbon.Appropriate activated-carbo concentration could obviously increase root rate.