Optimization of Enzymatic Extraction Gorgon Nut Protein Using Response Surface Methodology

Composite-enzymolysis in preparing protein of gorgon nut was optimized with response surface method.Effects of factors including the solid to liquid ratio，enzymolysis temperature，enzymatic content，enzymolysis pH，enzymolysis time with the extraction rates of gorgon nut protein were explored by single-factor and response surface experiment.Results showed that enzymolysis pH was the most critical factor affecting the extraction rate of gorgon nut，followed by enzymatic content，and enzymolysis temperature exhibited less effect on extraction rate of gorgon nut protein.The optimal enzymolysis conditions were the ratio of solid to liquid was 1∶20（gmL），enzymolysis temperature 49 ℃，enzymolysis pH 5.0，enzymolysis time 2 h，enzymatic content 0.35% and the extraction rates of gorgon nut protein was up to 80.38%.