Establishment of Rapid Propagation System for Platycodon grandiflorum

With Platycodon grandiflorum stem as explants，the regenerated plants were obtained by adventitious buds inducing and rooting.Callus induction and differentiation were studied using stems and leaves from aseptic seedlings as explants.Results showed that optimal medium for adventitious buds induction of stems was MS + 6-BA 1.0mgL + NAA 0.05mgL.The test-tube plantlet could root in the medium 12MS +IAA 1.0mgL+NAA 0.5mgL.Callus that were induced from two kinds of explants and inductivity of stem explants was the highest in medium MS + 2，4-D 1.0mgL+6-BA 0.2mgL.All kinds of explants from callus were differentiated.The rapid proliferation of Platycodon grandiflorum by tissue culture method could be established，which would provided effective ways for the breeding of excellent varieties and utilization of Platycodon grandiflorum.