Test and Research on Virus-free Rapid Propagation Technology and Micro Bud Grafting Technology of Citrus

Using Citrus sinensis Osbeck cv.Hongjiang and Citrus Shatangju，virus-free adventitious buds were formed by inducing culture.Complete non-toxic plants were cultured by grafting seedling to rootstock seedling.Test results showed that success rate of explant sterilization was 86%，optimum medium of induction of adventitious buds was MT+6-BA 3.0 mg/L+ IBA 0.1 mg/L+KT 0.1 mg/L.Optimum medium of adventitious bud subculture was MT+6-BA 2.0 mg/L+IBA 0.1 mg/L and proliferation coefficient was 2.3.Seedling rate of stock seed reached 90%.Average survival rate of micrograft reached 87%.This study provided a new way of rapid propagation for Citrus virus-free seedling.