Separation Purification and Free Radical Scavenging Activity of Polysaccharide from Flammulina velutipes Germanium Riched

Flammulina velutipes strain was used as research material，germanium enriched hyphae was obtained by fermentation technology，and polysaccharides were separated and purified by DEAE cellulose and G-100 chromatography column.Antioxidant activity of germanium enriched Flammulina velutipes polysaccharide in vitro was studied.Experimental results showed that，a single component polysaccharide was obtained by separation and purification，purity 99.78%，nonprotein.Ge content in mycelium was 7.42 mgg，percent conversion of organic Ge was 6.25%.Antioxidant ability enhanced significantly of polysaccharide purified and effectively scavenged oxygen free radical，hydroxyl radical and DPPH，they had significant difference compared with blank control.Scavenging rate of free radical increased with concentration of polysaccharide Ge enriched.When the polysaccharide concentration was 900 μgmL，scavenging rate of oxygen free radical，hydroxyl radical and DPPH was 68.16%，64.26%，64.3%，respectively.The polysaccharides Ge-riched had potential to developed into a natural antioxidant，functional foods or drugs.