Propagation Experiment of Pholidota cantonesis Rolfe.
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Graphical Abstract
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Abstract
Regeneration system of Pholidota cantonesis Rolfe. was studied by taking Pholidota cantonesis Rolfe. legume as explants.Results showed that impregnation with 0.1% HgCl2 for 8 min was beneficial to asepsis seeding for Pholidota cantonesis Rolfe. legume and had low pollution rate.When seed germination medium was MS+0.5 mg/L BA+0.05 mg/L NAA+7 g/L agar,germination rate of 72%,PLB color was green and agglomerate.When induced proliferation medium was MS+0.1 mg/L ZT+30 ml/L coconut+0.05 mg/L NAA+7 g/L agar,induction rate of 70%,maximum increment coefficient was 4.11.When seed germination medium was 1/2MS+50 g/L potato+50 ml/L banana juice+1 g/L peptone,strong seedling effect was the most obvious,maximum width of seedlings was 3.54 mm,and plants were relatively thick.
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