Effect of Medium on Germination of Pholidota chinensis Lindl Seeds

Pholidota chinensis Lindl is the main source of headache medicine.Research of Pholidota chinensis Lindl seed germination can provide technical support for the development of pharmaceutical industry.Pholidota chinensis Lindl seeds with 80% maturity were used as experimental materials.Effects of different concentrations of auxin，hormone and additives on seed germination and growth were analyzed.Results showed that the optimum medium for seed germination was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+KT 0.5 mg/L+peptone 1 g/；optimum medium for protocorm proliferation induction was MS+6-BA 1.0 mg/L+KT 0.5 mg/L+NAA 0.5 mg/L+peptone 1 g/L；optimum medium for pseudobulb induction was 1/2MS+peptone 1.5 g/L+banana mud 40.0 g/L+potato mud 40.0 g/L+Huabao 1.0 g/L。