Application of Experiment on Proliferation Effect of Prebiotics on Probiotics in Undergraduate Teaching

Taking two prebiotics fructooligosaccharides（FOS）and isomaltooligosaccharides（IMO）as carbon source of culture medium，sugar free medium and ordinary MRS medium as negative and positive controls，effect of oligosaccharide prebiotics on proliferation of L.casei was investigate.Results showed that both FOS and IMO could promote proliferation of L.casei，and prebiotic effect of FOS was better than IMO.Effect of FOS on proliferation of probiotics was further explored by adding different concentrations of FOS as carbon source in sugar free medium.Results showed that proliferation of L.casei increased gradually with increase of FOS concentration.When FOS concentration was higher than 2%，continuous increase of concentration did not significantly promote proliferation of L.casei.This experimental content was open and exploratory.And it switched traditional “curriculum centered”experimental teaching to “problem centered”scientific research teaching.Application of experimental items in undergraduate teaching was summarized from selection of teaching cases，implementation process of experimental projects and discussion of experimental results.It provided reference for teaching reform of similar courses.