Abstract: Control effect of spraying biogas slurry with different concentration on grape leaf hopper and grape downy mildew was studied．There were 6 treatments in field and greenhouse experiments．Inoculation test of downy mildew pathogen and observation test of leaf hopper harm was carried out by spraying biogas slurry with different concentrations from grape leaf opening stage．Experimental results showed that different concentrations of biogas slurry spraying had a certain effect on the harm of grape leaf hopper．Compared with T₀ without biogas slurry spraying, harm of exposed land leaf hopper was significantly reduced, and gradually decreased with the increasing of biogas slurry spraying concentration．The harm rate of biogas slurry spraying leaf hopper was 17.40%, and the control effect was 49.12%．Spraying grape leaves with different concentration of biogas slurry has the control effect on grape downy mildew in different cultivation environments （open field and greenhouse）, and 80% biogas slurry has the most significant control effect, which was 58.54% and 50.56%, respectively．Results revealed role of biogas slurry in the control of grape diseases and insect pests, which was conducive to comprehensive utilization of biogas slurry in grape cultivation．