Abstract: To better cultivate comprehensive talents and break isolation between different traditional experimental courses, extraction and determination of folic acid were brought into experiment course of food biochemistry for undergraduates for the first time．Based on ordinary food as raw material, folic acid was extracted by enzymolysis and detected by microbial method．Combination courses of Food Biochemistry and Microbiology will extend students' experimental knowledge by a large margin and help them to build up integral theoretical system of Food Biochemistry and Microbiology．This experiment course, with low cost, could be adopted as a comprehensive experiment project of food specialty in colleges and universities．