Abstract: Taking food analysis experiment course as an example, reform and practice of undergraduate experimental teaching assessment system under background of new engineering was expounded．In view of shortcomings of current experimental assessment and evaluation system, on the basis of fully reflecting characteristics of food science and engineering, combining characteristics of curriculum and experimental system, and according to different objectives and requirements of different types of experimental projects, a diversified experimental assessment and evaluation system was established．The practice of three sessions of students has proved that assessment system was conducive to guiding students to learn independently, objectively and fairly reflecting students' actual experimental level, and stimulating students' enthusiasm and interest in learning．Assessment and evaluation system focused on student development, which was conducive to improving quality of talent training．The project was worth further promotion and research.