Abstract: At present, domestic liquid inoculator of edible fungus bag planting has only manual and semi-automatic models, with low production efficiency, poor accuracy of inoculum and high pollution rate, which can not meet requirements of large-scale factory production of edible fungus bag planting． According to technical requirements for production of liquid inoculation of edible fungi in bags, research and development of automatic continuous operations such as automatic transportation, positioning inoculation and detection of liquid inoculation of edible fungi were carried out． Problems of difficult location of flexible inoculation, uneven inoculation amount, high pollution rate were analyzwd． R & D design scheme of large-scale production of edible fungus bag planting liquid inoculum machine was put forward． It could greatly improve inoculation quality and production efficiency, and promote transformation, upgrading and sustainable development of China's edible fungus industry．