Abstract: Experimental teaching is a necessary way for students to understand and master theoretical knowledge, which helps to improve students' interest in learning and cultivate their practical ability．Agriculture related majors of College of Plant Sciences include agronomy, plant protection, horticulture, resources and environment．Mechanical courses of these majors have less experimental hours, less but incomplete equipment, obsolete equipment and weak pertinence．In view of above situation, three sets of self-made teaching aids were developed, which have been successfully applied in mechanical experiment course and open innovation experiment of four agriculture related majors in College of Plant Sciences．In experimental teaching, the "mixed teaching mode of introducing self-made teaching aids" was constructed, which was very popular with students．Through a questionnaire survey among students, students' improvement or recognition of seven questions, such as teaching mode, improving learning interest, self-study ability and applicability of self-made teaching aids, were more than 90%．Self-made teaching aids and its mixed teaching mode have greatly improved classroom efficiency and teaching quality．