Abstract: Taking Chinese cabbage "Beijing New No.3" as test material, using different specifications of acupuncture tray seedlings, four treatments of 50, 72, 105 and 128 holes/plate were set up to explore influence of number of holes on growth quality, yield and quality of Chinese cabbage, and optimal treatment was screened．Results showed that number of holes in seedling tray affected yield and quality of Chinese cabbage by influencing growth index of Chinese cabbage seedling stage．With increase of number of holes in acupuncture tray, yield of Chinese cabbage with 50 holes/plate tray seedlings was the best after transplanting, and its biological yield and economic yield were 3.09%, 34.03%, 38.73% and 33.73%, 42.47% and 45.70% higher than those of Chinese cabbage with 72, 105 and 128 hole tray seedlings respectively．Yield and content of soluble sugar and vitamin C were 12.02%, 13.26%, 40.41% and 0.83%, 15.84% and 22.31% higher than those of Chinese cabbage grown in hole trays of 72, 105 and 128 holes respectively．50 hole and 72 hole trays were better seedling specifications for Chinese cabbage．