Abstract: Practical teaching reform is an important part of construction of new agricultural sciences, and online teaching model has also been widely used in recent years．In order to better apply online teaching mode in practical teaching of new agricultural science, taking practical course of Crop Cultivation and Tillage as an example, combined with recent online teaching insights, problems faced by current online teaching mode in practical teaching of new agricultural science were analyzed, and impact of online teaching mode on practical teaching of new agricultural science was elaborated．Reform measures have been proposed from four aspects: teaching methods, teaching abilities, classroom format, and assessment models．