Abstract: Plant factories are used in scientific research and plant production with advantages of high production efficiency, high automation, independent of natural conditions and adjustable environment．However, plant factories still have problems such as laborious cultivation plate cleaning and incomplete cleaning, which not only affect plant growth, but also restrict production efficiency of plant factories．Compared with existing facility agriculture cleaning methods, working principle of plant factory cultivation board cleaning was studied, and a plant factory cultivation board cleaning device based on high-pressure water jet technology was designed using Auto CAD software．Cleaning structure, nozzle type, and water jet influencing factors（water jet cone angle, water jet pressure, target distance, and water jet velocity）were studied, and optimal parameters for plant factory cultivation board cleaning device was determined, which were water jet target distance from 20 cm to 60 cm, water jet cone angle of 65 °, and water jet pressure of 1000 Pa, and cleaning nozzle type was a fan-shaped nozzle with a diameter of 3.5 mm．Cleaning effect of cultivation boards was predicted and simulated using Soilworks software．Experimental results showed that cleaning device based on high-pressure water jet could effectively clean cultivation board of plant factory and improve work efficiency of plant factory．It could provide theoretical basis and technical support for research of cultivation board cleaning devices, and provide reference for design and development of cleaning devices in plant factory．