Abstract: In order to explore effect of cold plasma seed treatment technology on grafting seedlings survival rate and later growth of forage bitter melon, effects of cold plasma seed treatment on wound healing, survival rate, growth, resistance, and commercialization of feed bitter melon rootstock and panicle combinations were studied by using cold plasma technology．Days of isolation layer and callus formation, survival rate and growth data of treated feed melon gourd rootstock and panicle combinations, survival rate of feed bitter melon grafted seedlings, disease resistance of feed bitter melon, and pulp thickness of feed bitter melon were investigated, and data analysis were conducted．Results showed that appropriate technological treatment of stock and scion combinations could shorten healing time by 1 to 2 days, significantly increase scion survival rate, increased scion growth by 1.17% to 7.62%, and increased peel thickness by 1.75% to 8.77%．It overcomed shortcomings of existing forage bitter melon such as slow healing of grafted wounds and secondary wound infection．After transplantation, plant incidence rate was reduced, stress resistance was enhanced, and commerciality was improved, so it has good use and promotion value．