Abstract: Crop germplasm resource bank is a storage facility for preservation of crop germplasm resources, including low temperature seed bank, in vitro library, ultra-low temperature library, DNA library and so on．Construction of crop germplasm resource bank is highly professional and involves a wide range of professions．Moreover, crop germplasm resource bank needs to operate safely and stably for a long time, and safety and reliability of facilities and equipment are extremely high．Therefore, its design and construction are particularly important．Taking construction of Guangxi crop germplasm resource bank as an example, construction content, parameter design, installation and construction, and advanced nature of crop germplasm resource bank were explained．It focused on parameter design and installation and construction requirements of repository body．Four suggestions were put forward in order to serve as a reference for construction of new crop germplasm resource bank throughout the country．