Abstract: A fully automatic cleaning machine for inner and outer walls of plant tissue culture bottles had been developed according to requirements of cleaning process for plant tissue culture bottles, in response to current problems of low cleaning efficiency, high labor intensity, and high usage cost in production of factory grown seedlings．Key components had been designed and tested, appropriate operating parameters had been determined．Experiment showed that, when spray and brush cleaning time was 4 times, cleaning time was 3 seconds, and bottle speed was 150 r/min, average cleanliness and breakage rates of cleaning machine were 93.33% and 0.83%, cleaning efficiency was 960 bottles/h．The fully automatic cleaning machine developed could efficiently clean inner and outer walls of plant tissue culture bottles, meeting needs of factory production．