Abstract: In response to shortage of water and land resources in China, an optimized allocation model for water and land resources in irrigation areas was constructed．This model was of great significance for ensuring efficient and green development of irrigation districts and implementation of sustainable agricultural development strategies．Taking socio-economic development and ecological requirements of irrigation district into account, an optimization model was established with goal of maximizing efficiency of water use per square meter and ecological green equivalent based on multi-objective genetic algorithm（NSGA-II）．Taking Lailong Irrigation District of Jiangsu Province as an example, optimal allocation plans for water and land resources with the greatest socio-economic benefits, ecological benefits, and cultivated land increase rate were proposed．Results indicated that when aiming to maximize social and economic benefits or increase rate of cultivated land area, main means of the Lailong Irrigation District was to transform water bodies and water conservancy facilities into cultivated land, urban villages, and industrial and mining land．Proportion of water distribution for water bodies and water conservancy facilities in irrigation district decreased, while proportion of water distribution for cultivated land, urban villages, and industrial and mining land increased．Unilateral water use efficiency increased by 9.85% and 0.54% respectively, but ecological green equivalent decreased by 5.52% and 0.98%．When the goal was to maximize ecological benefits, land consolidation should be carried out to transform farmland or facility agricultural land into garden grasslands, water bodies, and water conservancy facilities．Proportion of water resource allocation in irrigation areas should be the same as current situation．Unilateral water use efficiency and ecological green equivalent have increased by 0.26% and 0.38% respectively．Water demand of three optimized configuration schemes has increased, but it has not exceeded available water supply of Lailong Irrigation District．This study could provide effective decision-making support for ecological construction in irrigation areas and sustainable agricultural development．