破布叶组培快繁技术

Tissue culture and rapid propagation technology of Microcos paniculata L.

摘要: 以破布叶茎段为外植体，通过植物组培快繁技术，培养成完整的脱毒植株。试验结果表明，外植体灭菌处理成功率94%，诱导不定芽的最适培养基1/4MS+0.5 mg/L 6-BA ；不定芽继代增殖的最适培养基1/4MS+0.5 mg/L 6-BA+ 0.1 mg/L NAA+0.1 mg/L GA₃ ，增殖系数3.3；最适出根培养基1/4MS+1.0 mg/L IBA+ 0.1 mg/L NAA，出根率93.1%；组培苗移栽成活率98%，为破布叶种苗提供了一种新型快速的繁殖方式，将促进岭南特色中药材破布叶高效新型产业化的形成和发展。

Abstract: Using stems of Microcos paniculata L. seedlings as explants, complete and virus-free plants were obtained by tissue culture and rapid propagation technology．Results showed that explants sterilization success rate was as high as 94%．Optimal medium for adventitious buds induction was 1/4MS+0.5 mg/L 6-BA．Optimal medium for subculture multiplication was 1/4MS+ 0.5 mg/L 6-BA+ 0.1 mg/L NAA+0.1 mg/L GA₃ , and subculture multiplication factor was up to 3.3．Optimal medium for root was 1/4MS+1.0 mg/L IBA+0.1 mg/L NAA, while rooting rate was 93.1%．Transplanting survival rate was averaged at 98%．It could provide an effect way for rapid propagation of virus-free seedlings, which would promote formation and development of high efficiency and new industrialization of Microcos paniculata L., a characteristic Lingnan medicinal plant．