Abstract: To investigate effects of humic acid water-soluble fertilizer on greenhouse strawberry growth, development and quality, a field experiment was carried out with "Red Cheek" strawberry as material．Results indicated that one week after applying humic acid fertilizer, average plant height and crown diameter of strawberry plants increased by 60% and 62%, compared to control group treated with plain water．Continuous monitoring of leaf SPAD values showed a significant increasing trend, consistently higher than control group．Soluble solids and sugar-acid ratio in fruit increased by 9% and 20%, compared to control group, reaching a significant level, and improving fruit taste．Applying humic acid fertilizer also effectively increased fruit hardness, facilitating commercial transportation．Application of humic acid fertilizer could also increase available nutrient content of soil such as organic matter, alkali-hydrolyzed nitrogen, available potassium, and available phosphorus．In summary, after applying humic acid water-soluble fertilizer, physical and chemical properties of soil were improved, strawberry plants growth was more vigorous, promoting synthesis of chlorophyll in strawberry leaves, enhancing plant's photosynthetic rate, to provide sufficient dry matter accumulation for growth of strawberry fruits．This led to an improvement in nutritional and sensory quality of strawberry fruits, effectively achieving increased productivity and efficiency．