Abstract: To establish tissue culture and rapid propagation technology system for Amorphophallus konjac in Bijie City, to promote high-quality development of Amorphophallus konjac industry, tissue culture technology research was conducted using rhizomes of Amorphophallus konjac as explants．Results indicated that an optimal disinfection combination was 30 s in 75% alcohol + 10 minutes in 0.1% HgCl₂, which yielded the highest survival rate of 87.30%．Different exogenous hormones had varying effects on induction of callus, adventitious buds, and roots．Among these, medium MS +1.0 mg/L 6-BA + 1.0 mg/L NAA was more suitable for callus differentiation, with the earliest differentiation time, highest induction rate, and greatest weight gain．The highest rate of adventitious bud induction was medium MS + 1.0 mg/L 6-BA + 0.8 mg/L NAA, which also produced higher number of bud points per callus segment, averaging 6.85 buds．The highest proliferation coefficient of clustered buds was medium MS+ 0.8 mg/L 6-BA + 0.8 mg/L NAA, showing the best growth．Medium 1/2 MS+ 0.50 mg/L IBA was the most favorable for root induction．A substrate composition of 30% coconut coir + 50% sandy soil + 20% vermiculite was optimal for acclimatization and transplantation of konjac tissue culture seedlings．A tissue culture and rapid propagation technology system of Amorphophallus konjac in Bijie City was established, and seedlings were successfully obtained acclimatization and transplantation．