Abstract: Aiming at poor water retaining capacity and fertilizer retention capacity of aeolian sandy soil in southern Xinjiang Uygur Autonomous Region, effects of different nitrogen application rate on tomato growth, yield and quality in aeolian sandy soil were carried out．Five treatments were set up with nitrogen application rates of 0, 200, 350, 500, and 650 kg/hm², and plant height, stem diameter, root characteristics, leaf photosynthetic characteristics, soil nitrate-N accumulation, yield, and quality of tomato were measured．Results showed that with increasing nitrogen application rates, stem diameter, net photosynthetic rate, root length, root volume, and root surface area first increased and then decreased, with all reaching their maximum values at a nitrogen application rate of 500 kg/hm²．Soil nitrate content increased with increasing nitrogen application rate, and was significantly higher at 650 kg/hm² than at other treatments．Tomato yield and soluble sugar content increased first and then decreased with increasing nitrogen application rates, with the highest yield observed at 500 kg/hm²．Soluble protein and soluble solids content increased with increasing nitrogen application rates, reaching their maximum values at 500 kg/hm²．Considering soil fertility conditions of aeolian sandy soil in southern Xinjiang, optimal recommended nitrogen fertilizer application rate for tomatoes was 350～500 kg/hm²．