中国农业机械化科学研究院集团有限公司 主管

北京卓众出版有限公司 主办

微波消解联合ICP-MS法测定不同产地海棠叶中重金属元素含量及形态分布

Content and speciation distribution of heavy metals in crabapple leaves from different habitats using microwave digestion and ICP-MS

  • 摘要: 为明确不同产地海棠叶中重金属元素(铅、镉与砷)含量及形态分布,选取重金属污染较重区域的云南省西府海棠、山东省垂丝海棠及河北省变叶海棠3个不同产地海棠叶作为样品,通过微波消解法对样品进行前处理,以浓硝酸、浓盐酸和过氧化氢为消解试剂,设定消解程序,利用微波消解仪消解样品。根据铅、镉、砷元素在ICP-MS标准溶液中的响应强度测定各重金属元素含量。采用BCR提取法对重金属元素的4种赋存状态(酸可提取态、可还原态、可氧化态和残渣态)进行提取,并计算各形态含量占比。结果表明,产地环境因素差异对海棠叶中重金属元素含量及形态分布具有显著影响,并且主要与土壤性质和污染状况相关。其中,河北省变叶海棠中铅、镉、砷3种重金属元素含量分别为0.25、0.08和0.12 mg/kg,均明显高于其他两地;云南省和山东省海棠叶中3种重金属元素有效态含量占比均超过65%,具备较强二次释放潜力。研究成果为评估海棠叶品质安全及产地环境质量提供科学依据,有利于提升海棠叶药用价值。

     

    Abstract: To clarify content and speciation distribution of heavy metals(such as lead, cadmium, and arsenic)in crabapple leaves from different geographical origins, crabapple leaf samples were selected from crabapple species including Yunnan Province(Malus×micromalus Makino), Shandong Province(Malus halliana Koehne), and Hebei Province(Malus bhutanica(W. W. Sm.)J. B. Phipps), all sourced from regions with relatively high heavy metal pollution.Samples were pre-treated using microwave digestion.Concentrated nitric acid, concentrated hydrochloric acid, and hydrogen peroxide were used as digestion reagents.A digestion procedure was set, and samples were digested using a microwave digestion system.Contents of lead, cadmium, and arsenic were determined based on response intensity of these elements in ICP-MS standard solutions.BCR sequential extraction method was employed to extract four speciation forms of heavy metals(acid-extractable, reducible, oxidizable, and residual states), and proportion of each speciation form was calculated.Results indicated that significant differences in environmental factors across origins significantly influenced heavy metal content and speciation distribution in crabapple leaves, with these effects primarily related to soil properties and pollution levels.Specifically, contents of lead, cadmium, and arsenic in crabapple leaves from Hebei Province were significantly higher than those from the other two provinces, with specific values of 0.25, 0.08, and 0.12 mg/kg, respectively.In contrast, effective form content of three heavy metals in leaves from Yunnan and Shandong provinces both exceeded 65%, indicating a strong potential for secondary release.A scientific basis for evaluating quality and safety of crabapple leaves and environmental quality of their habitats was provided, thereby contributing to enhancement of their medicinal value.

     

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