蚕沙分离的溶磷解钾菌株OP6发酵配方

Fermentation Formula of Phosphate and Potassium-solubilizing Strain OP6 Isolated from Sericulture Excrement

摘要: 发酵是功能微生物菌株实用化的重要环节。以蚕沙中分离的耐高温、具溶磷解钾功能的巨大芽孢杆菌OP6菌株为目标菌株，采用单因素试验研究了其培养基中的碳源、氮源和金属离子等因子对OP6繁殖数量的影响，筛选获得最优发酵配方：玉米淀粉3%，豆粕粉3%，酵母膏0.9%，NaCl 2.5%，CaCO3 2.5%，MgSO4 0.4%，KH2PO4 0.3%，MnSO4 0.075%，pH值7.2~7.5。并对该配方进行了三角瓶和30 L发酵罐验证。

Abstract: Fermentation is an important step for application of functional microbial strains.The culture medium of OP6，one strain of Bacillus megaterium isolated from sericulture excrement with the function of phosphate and potassium-solubilizing，was studied by single factor experiment of carbon source，nitrogen source and metal ions.A optimal fermentation formula was：corn starch 3%，soybean meal powder 3%，yeast extract 0.9%，NaCl 2.5%，2.5% CaCO3，0.4% MgSO4，KH2PO4 0.3%，MnSO4 0.075%，pH 7.2-7.5.The formula effect was validated by experiments of tripod and 30 L fermentation tank.