HPLC法快速测定人参提取物中7种人参皂苷含量
Fast Determination of Seven Ginsenosides in Abstract of Panax Ginseng by HPLC
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摘要: 该文采用Kinetex核-壳色谱柱测定人参提取物中7种人参皂苷的含量。用甲醇超声波提取法提取样品，采用Phenomenex Kinetex C18 100A（50 mm×4.6 mm，2.6 μm）色谱柱，柱温常温，流动相乙腈：水，梯度洗脱，流速为1.8 mL/min，检测波长为205 nm，柱温为室温，对人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2和Rd进行定量分析。结果发现人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2和Rd的线性良好，重复性试验中人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2和Rd相对标准偏差为2.5%、2.9%、3.8%、1.5%、3.6%、1.9%和2.3%，人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2和Rd的加标回收率分别为102.6%、99.6%、98.7%、101.3%、99.2%、98.5%和101.8%。试验证明本方法快速、节省试剂，可用于人参提取物中人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2和Rd的含量测定。Abstract: The method for the determination of seven ginsenosides in abstract of Panax ginseng by Kinetex core-shell column was established．The separation was performed on Phenomenex Kinetex C18 100A（50 mm×4.6 mm，2.6μm）.The mobile phase consisted of acetonitrile（A）-water（B）with Linear gradient elution；at a flow rate of 1.8mL·min-1：the warelength of detector was 205nm.The column temperature was at room temperature.The calibration curve showed good linearity.The relative standard deviation of ginsenoside Rg1、Re、Rf、Rb1、Rc、Rb2 and Rd were 2.5%、2.9%、3.8%、1.5%、3.6%、1.9% and 2.3%.The recovery were 102.6%、99.6%、98.7%、101.3%、99.2%、98.5% and 101.8%.It has the advantage of simplicity，fast determination and less reagent consumed in determination of ginsenoside Rg1、Re、Rf、Rb1、Rc、Rb2 and Rd in abstract of panax ginseng.
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Keywords:
- Abstract of panax ginseng /
- Ginsenoside /
- HPLC
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