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QuEChERS-高效液相色谱法测定果蔬中多菌灵残留

QuEChERS-HPLC for Determination of Carbendazim Residues in Fruits and Vegetables

  • 摘要: 建立了一种以高效液相色谱(HPLC)测定果蔬中多菌灵残留的方法,该方法采用QuEChERS技术为前处理方法,以乙腈作为提取溶剂,以PSA(Primary Secondary Amine,N-丙基乙二胺)和C18填料作为净化材料。试验结果表明:多菌灵标准品在0.1~10.0 mgL范围内线性相关系数为1.000;方法定量限为0.1 mg/kg(SN=10);加标浓度为0.5和5.0 mg/L的平行5次测试结果相对标准偏差分别为2.98%和1.72%;0.1、0.5和5.0 mg/L 3个不同浓度的加标回收率在96%~110%之间。

     

    Abstract: A method was established for determination carbendazim residues in fruits and vegetables by HPLC,carbendazim was extracted by acetonitrile,PSA (primary secondary amine,N-propyl ethylenediamine) and the C18 filler was used as purification and this pre-treatment method was called QuEChERS method.The results showed the linear correlation coefficient of carbendazim standard was 1.0 in the range of 0.1~10.0 mgL,method limit of quantification was 0.1 mgkg (SN=10).When the spiked concentration was 0.5 and 5.0 mg/L,the relative standard deviations of five parallel tests with different concentration were 2.98% and 1.72%.Recoveries were in the range from 96% to 110% at the spiked level of 0.1,0.5 and 5.0 mg/L into samples.

     

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