Abstract: Growth of Bacillus subtilis BSn5 had been investigated in LB medium at different doses of Cr（VI）with or without hematite. Metabolic heat flux decreased with increasing of Cr（VI）concentration，indicated that subtilis BSn5 reduced their metabolic activity to enhance cellular detoxification ability.Cell growth and total thermal effect had been successfully employed simultaneously to assess the effect of Cr（VI）on Bacillus subtilis BSn5 in the absence and presence of hematite.Relative removal capacity and removal intensity of hematite for Cr（VI）were higher with Bacillus subtilis BSn5 than that without Bacillus subtilis BSn5，demonstrated that it was possible to reduce the toxicity of Cr（VI）in contaminated environment by applying a combined approach of hematite and Bacillus subtilis BSn5.