甜叶菊离体培养再生体系的建立

Establishment of Regeneration System in Vitro Culture of Stevia rebaudiana Bertoni

摘要: 以甜叶菊无菌苗的叶片、茎段为外植体，对甜叶菊离体培养及再生体系的建立进行了试验研究。结果表明：以茎段为外植体进行甜叶菊的组织培养优于叶片外植体。茎段外植体愈伤组织诱导及丛生芽增殖的最适培养基为：MS+0.5 mgL 6-BA+0.5 mgL NAA+3%蔗糖+0.7%琼脂；正交试验结果表明大量元素浓度对丛生芽的增殖影响最大，其次是6-BA、蔗糖，NAA的影响较小；生根最适培养基为MS+0.25 mgL IBA+0.2 mgL NAA+3%蔗糖+0.7%琼脂+0.3 mgL活性炭，添加低浓度活性炭的可明显提高生根率。

Abstract: Establishment method of tissue culture and regeneration system of Stevia rebaudiana Bertoni were studied by using leaves and stem as explants.Results indicated that stem used as explant was superior to leaf in tissue culture.Optimum medium for stem to induce callus and multiple shoots was MS+0.5 mgL 6-BA+0.5 mgL NAA+3% sucrose+0.7% agar.Based on orthogonal experiment，most important factor on inducing cluster buds was macroelement，next was 6-BA，sucrose，and the least was NAA.Optimum medium for rooting was MS+0.25 mgL IBA+0.2 mgL NAA+3% sucrose+0.7% agar+0.3 mgL activated carbon.Appropriate activated-carbo concentration could obviously increase root rate.