铁皮石斛组织培养技术

彭颖; 张莉芳; 程鹏; 谭萱; 洪亚辉

铁皮石斛组织培养技术

Tissue Cultivation Technique of Dendrobium officinale Kimura et Migo

摘要

摘要: 为构建铁皮石斛快繁体系，以铁皮石斛茎段、叶片为外植体，设计离体培养不同阶段的培养基配方。结果表明：使用70%乙醇进行表面灭菌处理30 s后，再用0.1%氯化汞溶液消毒9 min是最适宜铁皮石斛茎段的消毒方法；不同外植体对愈伤组织的诱导率有很大差异，以茎段诱导的愈伤组织出愈率最高，达到了83.3%；最适宜的不定芽诱导培养基为MS+0.5 mgL 6-BA+0.2 mgL NAA；适合铁皮石斛组培苗生根培养基为12 MS+0.5 mgL NAA。试验确定了最适培养条件，并成功获得了大量的铁皮石斛愈伤组织和幼苗，为铁皮石斛无性繁殖技术的研究提供了科学依据。

Abstract: To construct Dendrobium officinale Kimura et Migo rapid propagation system，taking stems segments and leaves of Dendrobium officinale Kimura et Migo as explant，medium formulas of culture in vitro in different stages were designed.Results showed that optimum disinfection method was surface sterilization treatment of Dendrobium officinale Kimura et Migo with 70% ethanol 30s and with 0.1% Mercuric Chloride 9 min.Different explants on callus induction rate had big differences，the highest rate of stem segments of callus induction reached 83.3%.Optimum adventitious bud induction medium was MS+0.5 mgL 6-BA+0.2 mgL NAA；and rooting was 12 MS+0.5 mgL NAA.Optimum culture condition was determined，and a lot of callus and seedlings of Dendrobium officinale were gotten.It provides a scientific basis for establishment of rapid propagation system of Dendrobium officinale Kimura et Migo.

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