Abstract: To make sure of exact effects of O3 water and O3 gas on greenhouse vegetables for disease and pest control，some experiments were tested under greenhouse condition，for instance，effects of crop seeds and soilborne disease on ozone water，and effects of control disease and pest of vegetable in greenhouse and damage levels on ozone gas.Results showed that metabolism and germinate should be promote by proper concentration of O3 water.O3 could quite inhibit at early stage the cucumber root rot，tomato bacterial wilt，pepper phytophthora blight，etc.The use of ozonegenerating equipment to the release of ozone gas in the greenhouse had a preventive effect on cucumber downy mildew，powdery mildew，angular leaf spot and gray mold and other airborne diseases.Release O3 every 10minutes for 10 times and 30minutes long every time，Bemisia tabaci and Liriomyza huidobrensis could be 100% killed completely under indoor controlled conditions.Indoor optimum O3 concentration is 08~10 mgm3.It is not only control disease and pest of greenhouse vegetable，but also no harm for growth and development of crop.