Abstract: With Gynura bicolor D.C stems as explants，the regenerated plants were obtained by adventitious buds inducing and rooting.Callus induction and differentiation were studied using roots，stems and leaves from aseptic seedlings as explants.The results showed the optimal medium for adventitious buds induction of stems is MS+6-BA1.0 mgL+NAA0.2 mgL+3.0% sugar.The test-tube plantlet can root in the medium 12MS+0~1.2 mgL NAA，and the rooting time is shorter with a higher concentration of NAA.Callus were induced from three kinds of explants and inductivity of root explants is the highest in medium MS+6-BA0.3 mgL+2，4-D 1.0 mgL+4.5%sugar.Only roots were differentiated from callus.