福建野生金线莲快速繁育技术

何碧珠; 何官榕; 黄铭星; 邹双全

福建野生金线莲快速繁育技术

Micropropagation Technology of Anoectochilus roxburghii in Fujian Province

摘要

摘要: 以金线莲茎段为试验材料，得到无菌的诱导芽体。通过萌发的芽体上的茎尖和茎段分别诱导愈伤组织和原球茎，建立金线莲的无菌培养体系，筛选出金线莲组培快繁的最佳培养基配方。结果表明：金线莲茎段诱导芽体萌发最适宜培养基配方为MS+BA 1.5 mg/L+香蕉汁100 g/L+NAA0.5 mg/L，茎尖诱导原球茎最适宜培养基配方为MS+BA 2.0 mg/L+NAA 0.3 mg/L+HyponcexⅠ3.0 g/L，原球茎继代增殖最适宜培养基配方为12MS+BA 2.0 mg/L+NAA 0.3 mg/L+香蕉汁100 g/L+AC 0.2％，愈伤组织诱导丛生芽最适宜培养基配方为12MS+BA 2.0 mg/L+NAA 0.2 mg/L+香蕉汁100 g/L，丛生芽继代增殖培养最适宜培养基为12MS+BA 3.0 mg/L+NAA 0.5 mg/L+KT 1.0 mg/L+香蕉汁100 g/L+AC 0.2％，幼苗生根培养最适宜培养基配方为12MS+IBA 1.0 mg/L+NAA 0.3 mg/L+AC 0.2％。

Abstract: Taking aseptic induced buds of Anoectochilus roxburghii stem as experimental material，callus and protocorm were induced form shoot tip and stem on germinal bud，respectively.Aseptic cultivate system Anoectochilus roxburghii of was established.And optimum medium for micropropagation was found.Results showed that optimum medium for bud germinate of Anoectochilus roxburghii stem was MS+BA 1.5 mgL+banana juice 100 gL+NAA 0.5 mgL.Optimum medium for protocorm induction of shoot tip was MS+BA 2.0 mgL+NAA 0.3 mgL+HyponcexⅠ3.0 gL.Optimum medium for subculture micropropagation of protocorm was 12MS+BA 2.0 mgL+NAA 0.3 mgL+banana juice 100 gL+AC 0.2%.Optimum medium for cluster buds induction from callus was 12MS+BA 2.0 mgL+NAA 0.2 mgL+banana juice 100 gL.Optimum medium for subculture micropropagation of cluster buds was 12MS+BA 3.0 mgL+NAA 0.5 mgL+KT 1.0 mgL+banana juice 100 gL+AC 0.2%.Optimum medium for rooting of plantlets was 12MS+IBA 1.0 mgL+NAA 0.3 mgL+AC 0.2%.

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