非洲紫罗兰愈伤组织诱导及植株再生

Callus Induction and Plant Regeneration of Saintpaulia ionantha

摘要: 以非洲紫罗兰为试验材料，以其无菌苗叶片为外植体，建立了愈伤组织诱导及植株再生体系。试验结果表明：MS+6-BA 2.0mg/L+NAA 0.5mg/L培养基配方诱导愈伤组织效果最好，出愈率可达73.9%，暗培养不利于愈伤组织诱导；在培养基MS+6-BA 1.0mg/L+NAA 0.1mg/L中愈伤组织分化不定芽的分化系数最高可达14.0；MS+NAA 0.5mg/L+IAA 0.5mg/L培养基可使生根率达到100%，平均单株生根数达12.4。

Abstract: Callus induction and plant regeneration system were constructed by using leaves of sterile Saintpaulia ionantha as explants.Results showed callus induction ratio were 73.9% and induction efficiency was best in the medium MS+6-BA 2.0mg/L+NAA 0.5mg/L.Darkness is not conducive to callus induction.Differentiation coefficient of callus was highest up to 14.0 in the medium MS+6-BA 1.0mg/L+NAA 0.1mg/L.Rooting percentage was up to 100% in MS+NAA 0.5mg/L+IAA 0.5mg/L medium，the average roots of each plant were 12.4.