Abstract: Physical and mechanical properties tests including measurement of stem diameter and seedling height under different seedling age were conducted，as well as seedling pull tests，extrusion and abruption tests of stem.It showed that stem diameter and seedling leaf height of rape Xiangza 1613 were approximately normal distributed simultaneously.Picking force showed a increase with the rise of location，a first decrease and a later increase with the rise of angle.Ability of extrusion and abruption resistance increased with time and that the best position on the bottom，best blade angle at about 50° and best transplanting for five age of seedling leaves.Results provided data and theory for designing a transplanting machine and further study on the key component structure，kinematic and kinetic parameters of rape transplanting machine，such as fetching seedlings，protecting seedlings and planting parameters.