Protamex酶解马铃薯蛋白粉制备抗氧化肽工艺优化
Optimization of Reparation for Antioxidant Peptide from Potato Protein Powder by Protamex
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摘要: 以马铃薯蛋白粉为原料，利用Protamex复合蛋白酶对其进行酶解制备抗氧化肽。以总抗氧化力为指标，采用Box-Behnken响应面设计对酶解工艺进行优化。结果表明，最佳酶解条件：底物质量浓度5%，加酶量4 000 Ug，酶解时间165 min，在此优化条件下得到的酶解产物的总抗氧化力为0.751 mmolL，回归得出的模型预测效果较好。Abstract: Antioxidant peptide was prepared by hydrolyzing potato protein powder with Protamex.With total antioxidant capacity as the indicator，the optimal technology was performed by means of Box-Behnken design.Results indicated that the optimal prepared technical parameters were obtained as follows：substrate concentration of 5％，enzyme dosage 4 000 Ug，enzymolysis time 165 min.Under the above mentioned conditions，the total antioxidant capacity was 0.751 mmolL.Experiment result was consistent with the prediction result.
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Keywords:
- Potato protein powder /
- Antioxidant peptide /
- Protamex /
- Response surface methodology /
- Enzymolysis
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