活性益生菌乳饮料复合稳定剂配方优化
Optimization of Compound Stabilizer Formula in Probiotic Milk Beverage
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摘要: 为提高益生菌乳饮料的稳定性,以益生菌乳饮料的离心沉淀率和感官品质为评定指标,在单因素试验确定各因素最佳添加量的基础上,通过正交优化试验研究了海藻酸丙二醇酯(PGA)、高脂果胶和低聚异麦芽糖对益生菌乳饮料稳定性的影响。正交优化试验结果表明,益生菌乳饮料复合稳定剂的最佳配比:PGA添加量0.03%,低聚异麦芽糖添加量1.8%,高脂果胶添加量0.012 5%。验证试验得到的产品离心沉淀率为2.38%,感官得分为89,质量指标理想。且在4 ℃条件下贮藏21 d后测定活菌数为7.93×109 cfumL,与无添加低聚异麦芽糖制得的益生菌乳饮料贮藏21 d后的活菌数3.21×108 cfumL相比,提高了一个对数级。Abstract: To improve stability of probiotic milk beverage,centrifugal sedimentation rate and sensory quality of probiotics beverage were used as evaluation index.Based on the single factor experiment for determining optimum addition amount of various factors,Propylene Glycol Alginate(PGA),high ester pectin and isomaltooligosaccharide were studied on the stability of brown probiotic milk beverage by orthogonal test.Orthogonal experimental results showed that optimal formulation of composite stabilizer was:PGA addition amount 0.03%,isomaltooligosaccharide addition amount 1.8% and high ester pectin addition 0.012 5%.Verification test showed that centrifugal sedimentation rate was 2.38 % and sensory score was 89.When stored under 4 ℃ after 21 d,the number of viable probiotics count remained at 7.93×109 cfumL.It raised a logarithmic compared with no added isomaltooligosaccharide of probiotic milk beverage that the number of living bacterium remained at 3.21×108 cfumL.
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