Abstract: With the seeds from 8 germplasms of energy crop Miscanthus which were cultivated in the nursery of Hunan Agricultural University as materials，their seed phenotypic traits，including seed length，width，thickness，ratio of length and width and 1000-seeds weight were investigated.The seed phenotypic differences between eight germplasms resources were studied by the methods of ANOVA analysis，multiple comparison，correlation analysis and hierarchical cluster analysis.The extremely significant differences were observed in the five traits between germplasms，the order of the mean variation coefficient of phenotypic traits for energy crop Miscanthus seed between different germplasms was：thickness＞ratio of lengthwidth＞width＞seed length＞1000-seed weight.Seed length had a significant positive correlation with seed width and 1000-seed weight.Seed width had an extremely significant positive correlation with seed thickness and 1000-seeds weight.Seed thickness had an extremely significant positive correlation with 1000-seed weight.Ratio of seed lengthwidth had a negative significant correlation with seed width，thickness and 1000-seed weight.The eight germplasm resources of energy crop Miscanthus were classified into three groups by using hierarchical clustering analysis.