Abstract: Walnut in Yunnan province is an important economic tree species.Speeding up development of walnut industry is an important part of construction of ‘a green economy strong province’ and new socialist countryside.Walnut germplasm is rich in Kunming city，and potential is huge.Foreign varieties planted in Kunming are highly affected by environment and soil.Excellent germplasm resources in local has not been explored and utilized well.Shortage of seed and seedling seriously restricted and affected the health，sustainable，rapid development of walnut industry，which has become a pressing matter of the moment for development of walnut industry in Kunming city.According to natural and geographical status of Kunming city，optimizing staff went deep into main planting walnut county （city） area in Kunming city for on-the-spot investigation.Through recommendation of township farmers，primary beads were chosen based on investigation and comparison.Adaptive test on primary beads were taken to find good single plant eventually.It is in order to promote healthy development of walnut industry in Kunming city.