Abstract: Through the research and development of physical disinfection equipment and technology for seedling materials and tobacco leaf-cutting machine，the aim was to disinfect seedling materials and cutting tools.At the same time，greenhouse health promotion and disease prevention electric defogging system and electrostatic zappers were used to reduce tobacco seedling diseases and insect pests，and promote growth of tobacco seedlings.Test results indicated that，health promotion and disease prevention electric defogging system played a role on promoting root growth and tobacco seedlings growth.Electrostatic extermination system of 2DJ-200 could effectively reduce pest quantity in various regions.The atomic oxygen spray system could inhibit growth of TMV、CMV、PVY and Botrytis，and it had a direct relationship on incidence of tobacco field.Diseases and pests physical control of float breeding have no effect on tobacco field growth.