Abstract: The possible effects of Ca2+，EGTA，DPI on protective enzyme and NADPH oxidase of Lonicera macranthoides Hand.-Mazz seedlings under 200 mmolL NaCl stress in solution culture were studied and a common cultivar“Yulei No.1”was used.Results showed that，Ca2+ increased SOD，CAT and NADPH oxidase activity were more significant than ABA，EGTA significantly inhibited the activity of CAT，SOD and NADPH oxidase of the Lonicera macranthoides Hand.-Mazz.The activity of CAT，SOD and NADPH oxidase did not recover under EGTA+ABA treatment.It suggested that the ABA had no significant effect on improving the activity of protective enzyme in the absence of lacking Ca2+.DPI significantly inhibited the activity of CAT，SOD and NADPH oxidase of the Lonicera macranthoides Hand.-Mazz.And the activity of CAT，SOD and NADPH oxidase did not recover under EGTA+ABA treatment.