Abstract: Nutrients contained in pumpkin have a negligible effect for prevention and treatment of human diseases.Pectin，cyclopropyl amino acids and trace elements zinc and chromium contained in pumpkin play a major role in the prevention of diabetes.For a long time，as people lack of awareness of the nutritional value of pumpkin，only part amount of pumpkin were eaten，and part of pumpkin were used as feed，as well as causing insufficier usage of pumpkin resources.In this paper，the contents of Cr in 200 pumpkin resources were determined by dry ashing-atomic absorption spectrometry.Sample number 203 has the lowest chromium content 0.015 3 mg/kg.Sample number 233 has the highest chromium content 0.520 0 mg/kg，which has exceeded the national limited standard（≤0.5 mg/kg）.200 germplasm materials were cluster analyzed，in which were screened out varieties with suitable content chromium offer technology and germplasm basis for breeding new varieties in rich-chromium pumpkin.